Natrium muriaticum Anhang Comparison

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http://ir.dut.ac.za/bitstream/handle/10321/47/Hofmeyr_2004.pdf?sequence=8

[Dorita Hofmeyr]

A nuclear magnetic resonance study of potencies of natrum muriaticum 15CH prepared by trituration and succussion versus natrum muriaticum 15CH prepared by succussion alone.

ABSTRACT

The purpose of this investigation was to analyse and compare the Nuclear Magnetic Resonance (NMR) spectra of potencies of Natrum muriaticum 15CH prepared by trituration and

succussion, and Natrum muriaticum 15CH prepared by succussion alone. It was hypothesized that in terms of the effect of trituration (or not) of these substances that significant

differences exist between the chemical shift and relative integration values of the CH2, CH3, H2O and OH signals of these homoeopathic substances. It was further

hypothesized that the process of trituration plays an integral part in the development of distinct physicochemical identities in the potencies mentioned above.

The investigation was designed as a scientific experiment whereby centesimal potencies were prepared according to the directions of H. to the 15CH level. Volumes of 15ml of the final

liquid potencies (87% ethanol) of each group were prepared and sent for analysis.

NMR spectroscopy was conducted on three samples of each sample group.

The samples were drawn into coaxial sample tubes making use of acetone as an external lock and using ethanol as the reference. The samples were drawn by the resident NMR-technician

in the Department of Chemistry, University of KwaZulu-Natal, Pietermaritzburg. The NMR spectrometer used was a Varian 500MHz INOVA having a 5mm broadband switchable probe

and a 5mm inverse detection probe. The pulse angle was set at 90° and the emperature was maintained at a constant value of 298.1 K (25° C).

The data was recorded and expressed in the form of NMR spectra giving the chemical shift value and integration values of the peaks. The chemical shift and relative integration values of the

CH2, CH3, H2O and OH signals were subjected to a process of statistical analysis using two main steps. The two sample groups were statistically compared by applying the t-test to the

chemical shifts and the ANOVA method to the relative integrations. The level of significance was set at α = 0.05 for all test comparisons.

Statistically significant differences were noted in the t-test comparison of the chemical shift values for the H2O and OH signals. No statistically significant differences were noted for CH2

or CH3 signals. For the ANOVA method of comparison of relative integration statistically significant differences were found to exist for CH2, H2O and OH signals. There were no statistically

significant differences observed for CH3 signals.

The result of the study did not allow a conclusive explanation of the specific structures responsible for homoeopathic remedy action.

However the results did serve to support the hypothesis that the effect of trituration (or not) on the substance render ethanol samples that are distinct from each other in terms of their identities measured

by NMR spectroscopy. It also serves to support the use of NMR spectroscopy as a tool in the investigation of the nature of homoeopathic potencies.

HYPOTHESIS

It is hypothesised that significant differences exist between the chemical shifts and relative integration values of CH2, CH3, H2O and OH signals of the two Natrum muriaticum 15CH potencies,

and that these indicate differences in the physical structure of the respective potencies.

CONCLUSIONS

It is apparent from the results of this investigation that the preparation of a remedy by the process of trituration yields a solution that is distinct in its physicochemical identity in terms of the NMR

spectra recorded. The study found that statistically significant differences exist in the t-test comparison of the chemical shift values. The ANOVA comparison of the relative integration values

also revealed statistically significant variation. It is thus clear that these findings are supportive of the hypothesis relating to the influence of trituration (or not) on the creation of distinct

physicochemical identities in the different test solutions.

However, a clear conclusion cannot be drawn regarding the influence of the action of trituration in this study. This does not preclude the possibility that this part of the pharmaceutical

procedure is important in the production of homoeopathic potencies.

It thus contributes further to the standardization of homoeopathic practice and manufacture. This study adds to the scientific data available for the assessment of homoeopathy.

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